Purification tags/Protein tag Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes. Affinity tags are appended to proteins so that they can be purified from their crude […]
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Identification of recombinants You are approaching the end of your cloning workflow. The DNA fragment was ligated in the vector of choice and transformed into E. coli cells. The transformed cells were streaked across a culture plate resulting in a multitude of colonies overnight. How do you know that all of the steps have resulted
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Bacterial Conjugation Conjugation is the direct transfer of DNA from one bacterial cell to another bacterial cell. The transferred DNA is a plasmid, a circle of DNA that is distinct from the main bacterial chromosome. The F plasmid is similar to a virus or a transposon in its ability to move independently of the main
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Cloning Vector A cloning vector is a DNA molecule in which foreign DNA can be inserted or integrated and which is further capable of replicating within host cell to produce multiple clones of recombinant DNA. Such a vector is called Cloning Vector. Plasmids and phages are the vectors used for cloning purposes, particularly in prokaryotes